| Unlockable: | How to unlock: |
| Roy | To unlock Roy, also from the Fire Emblem series, complete Classic Mode using Marth at any difficulty/stock. Roy will challenge you; defeat him and you can play as him. |
| Pichu | To unlock Pichu, complete Event 37 (Legendary Pokémon). Pichu will challenge you; win and you'll be able to play as Pichu! |
| Mr. Game & Watch | To unlock Mr. Game & Watch, you must complete both Classic or Adventure 1-P mode or the Target Test with ALL characters at any difficulty/stock, regular and hidden (that includes Mewtwo). After you do that, you'll be challenged; win this battle. |
| Mewtwo | To unlock Mewtwo, play in VS. Mode for either 20 hours (Combined VS. Play Time total), or play 700 matches in that mode. After either requirement is met, Mewtwo will challenge you; beat him. |
| Young Link | To unlock Young Link, complete Classic mode with 10 different characters at any difficulty/stock. You'll then be challenged by Young Link; defeat him. |
| Marth - method 2 | Alternatively, you can simply use every character as a human player in VS. Mode, and get a challenge from Marth that way |
| Marth | To unlock Marth from the Fire Emblem series, play Classic mode with all 14 regular characters (not ones you've unlocked). Marth will then challenge you; beat him to gain him as a selectable character. |
| Luigi | To unlock Luigi, in Adventure Mode, complete the first stage with a 2 in the seconds meter (03:12:43 for example). You'll then view a scene where Luigi comes and kicks Mario away so he can fight you with Peach. Defeat them both, finish the game. Defeat luigi |
| Jigglypuff | To unlock Jigglypuff, simply complete the game in 1-P Mode at any difficulty/stock (Adventure or Classic). |
| Ganondorf | To unlock the evil Ganondorf, complete Event 29. You will then be challenged by Ganondorf; defeat him and you can play as him from then on. |
| Falco | To unlock Falco, you must complete the 100-Man Melee mode. Defeat Falco after you complete that, and you can play as him. |
| Dr. Mario | To unlock Dr. Mario, you must complete 1-P Classic Mode using Mario at any difficulty/stock and without losing |
