| Unlockable: | How to unlock: |
| Dry Bones | finish 50cc nitro grand prix any rank all gold trophies |
| Daisy | finish 50cc retro grand prix any rank all gold trophies |
| 3 Karts | finish 100cc nitro grand prix any rank all gold trophies |
| Waluigi | finish 100cc retro grand prix any rank all gold trophies |
| 7 Karts | finish 150cc nitro grand prix any rank all gold trophies |
| 150cc mirror engine class | finish all 50cc,100cc and 150cc any rank all gold trophies |
| R.O.B. | finish 150cc mirror nitro grand prix any rank all gold trophies |
| All Karts | finish 150cc mirror retro grand prix any rank all gold trophies |
| Mario Kart DS Original Main Screen | finish all 50cc,100cc,150cc and 150cc mirror engine class |
| Big Bully-Boss in level 1 missions | finish all level 1 missions |
| Eye Rock-Boss in level 2 missions | finish all level 2 missions |
| Goomboss-Boss in level 3 missions | finish all level 3 missions |
| King Boo-Boss in level 4 missions | finish all level 4 missions |
| Big Bob-omb-Boss in level 5 missions | finish all level 5 missions |
| Chief Chilly-Boss in level 6 missions | finish all level 6 missions |