| Unlockable: | How to unlock: |
| Naruto | Starter |
| Sasuke | Complete Mission: Kakashi Sensei's Test? |
| Sakura | Complete Mission: Kakashi Sensei's Test? |
| Shikamaru | Complete Mission: Kakashi Sensei's Test? |
| Ino | Complete Mission: Kakashi Sensei's Test? |
| Chouji | Complete Mission: Kakashi Sensei's Test? |
| Kiba | Complete Mission: Kakashi Sensei's Test? |
| Hinata | Complete Mission: Kakashi Sensei's Test? |
| Shino | Complete Mission: Kakashi Sensei's Test? |
| Neji | Complete Mission: Kakashi Sensei's Test? |
| Rock Lee | Complete Mission: Kakashi Sensei's Test? |
| TenTen | Complete Mission: Kakashi Sensei's Test? |
| Tsuande | Complete Mission: The Famous Kunoichi! |
| Orochimaru | Complete Mission: Super Villan! |
| Jiraiya | Complete Mission: Teach Him A Lesson |
| Gaara | Complete Mission: Protect Gaara! |
| Temari | Complete Mission: Rematch! |
| Kankuro | Complete Mission: I'll Handle This! |
| Kabuto | Complete Mission: Betrayal!? |
| Kakashi | Complete Mission: Secret Technique Training! |
| Guy | Complete Mission: Youth!! |
| Itachi | Complete Mission: Revenge! |
| Kisame | Complete Mission: Kisame's Counterattack! |
| Sakon | Complete Mission: Combo! |
| Tayuya | Complete Mission: Calculated! |
| Kidomaru | Complete Mission: Pinpoint Blindspot! |
| Jirobo | Complete Mission: Chubby's Pride! |
