Fulfill the following conditions to unlock CPU opponents for World Championship Mode. Teams not listed are either unlocked from the start, or unlocked by progressing through Story Mode.
| Unlockable: | How to unlock: |
| Ancient Gear Gadjiltron Dragon | Summon [Ancient Gear Gadjiltron Dragon] |
| Archlord Kristya | Beat "Green Baboon, Defender of the Forest" 3 times |
| Blackwing - Vayu the Emblem of Honor | Summon [Blackwing - Silverwind the Ascendant] |
| Blue-Eyes White Dragon | Beat "Darklord Desire" 3 times |
| Chaos Sorcerer | Beat "Solar Flare Dragon" 3 times |
| Crusader of Endymion | Activate [Mega Ton Magical Cannon] |
| Cyber Eltanin | Beat "B.E.S. Big Core MK-2" 3 times |
| Dark Simorgh | Beat "Great Shogun Shien" 3 times |
| Darkness Neosphere | Play for 100 hours |
| Destiny End Dragoon | Beat "Underground Arachnid" 3 times |
| Dragunity Knight - Gadearg | Beat "Ancient Fairy Dragon" 3 times |
| Earthbound Immortal Wiraqocha Rasca | Win with by using the effect of [Final Countdown] |
| Elemental Hero Absolute Zero | Beat "Fabled Leviathan" 3 times |
| Elemental Hero Neos | Beat "Ancient Sacred Wyvern" 3 times |
| Explosive Magician | Beat "Ally of Justice Decisive Armor" 3 times |
| Fabled Ragin | Beat "Evil Hero Dark Gaia" 3 times |
| Fossil Dyna Pachycephalo | Have 666 or more Summons |
| Garlandolf, King of Destruction | Play for 50 hours |
| Gigaplant | Summon [Blackwing - Silverwind the Ascendant] |
| Gladiator Beast Gyzarus | Summon [Gladiator Beast Heraklinos] |
| Gravekeeper's Visionary | Beat "Jurrac Meteor" 3 times |
| Green Gadget | Beat "Power Tool Dragon" 3 times |
| Harpie Queen | Win by using the effect of [Vennominaga the Deity of Poisonous Snakes] |
| Hundred Eyes Dragon | Beat "Ojama Yellow" 3 times |
| Judgment Dragon | Summon [Judgment Dragon] |
| Locomotion R-Genex | Summon [Flying Fortress SKY FIRE] |
| Lonefire Blossom | Beat "Gungnir, Dragon of the Ice Barrier" 3 times |
| Majestic Red Dragon | Summon [Majestic Red Dragon] |
| Naturia Beast | Beat "Reptilianne Vaskii" 3 times |
| Raiza the Storm Monarch | Beat "Lava Golem" 3 times |
| Stardust Dragon/Assault Mode | Unlock 50% or more Duel Bonuses |
| Supersonic Skull Flame | Beat "Mist Valley Apex Avian" 3 times |
| Swap Frog | Beat "The Dark Creator" 3 times |
| The Immortal Bushi | Beat "Naturia Landoise" 3 times |
| Worm Zero | Highest damage is 10000 or more |
| XX-Saber Hyunlei | Win 10 duels in a row |
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