Mistress Spencer:
Successfully clear Story mode using Miss Spencer

Noble Rose:
Successfully clear Story mode using Evil Rose

Rowdy Reiko:
Successfully clear Story mode using Reiko Hinomoto

Sergeant Clemets:
Successfully clear Story mode using Dixie Clemets

Unlock Becky:
Successfully clear Story mode using Candy Cane

Doctor Cutter:
Successfully clear Story mode using Anesthesia

Judgment:
Successfully clear story mode using Bloody Shadow

Killer Khan:
Successfully clear Story mode using Aigle

Showbiz:
Successfully clear Story mode using Aisha

The BBD:
Successfully clear Story mode using Makoto Aihara
Unlock Gallery Mode:
Win the title with any girl

Ulock Beach Mode:
Defend your title with any girl
Unlock Lady X
Beat Story mode with every character to unlock Lady X. Beat it with their alter ego to unlock Lady X Substance.