| Unlockable: | How to unlock: |
| Technical Difficulties (Bronze) | Objective: Complete Spain Sequence 1 |
| Battle Wounds (Bronze) | Objective: Complete DNA Sequence 1 |
| Sanctuary! Sanctuary! (Bronze) | Objective: Complete Present Sequence 1 |
| Requiescat In Pace (Bronze) | Objective: Complete Spain Sequence 4 |
| Perfect Recall (Bronze) | Objective: Get a Sequence to 100% Memory Synchronization |
| Déjà vu (Bronze) | Objective: Replay a Memory |
| Undertaker 2.0 (Bronze) | Objective: Complete LGS 1 [Catacombs] |
| Golden Boy (Bronze) | Objective: Complete LGS 2 [Trajan Bath] |
| Bang! (Bronze) | Objective: Destroy the Machine Gun |
| Splash! (Bronze) | Objective: Destroy the Naval Cannon |
| Boom! (Bronze) | Objective: Destroy the Bomber |
| Kaboom! (Bronze) | Objective: Destroy the Tank |
| Home Improvement (Bronze) | Objective: Upgrade 5 buildings in the Antique district |
| Tower Offense (Bronze) | Objective: Kill All the Borgia Leaders |
| Show Off (Bronze) | Objective: Complete 10 Faction Creed Challenges |
| Perfectionist (Bronze) | Objective: Get 3 Gold Medals in VR Missions |
| Brotherhood (Bronze) | Objective: Recruit 3 Assassins |
| Welcome to the Brotherhood (Bronze) | Objective: Have a Trainee reach the Rank of Assassin |
| Capture the Flag (Bronze) | Objective: Remove All Borgia Flags in Rome |
| In Memoriam (Bronze) | Objective: Collect All Feathers |
| Dust to Dust (Bronze) | Objective: Find 1 Artifact in the Present |
| Serial Killer (Bronze) | Objective: Perform a 10 combo-kill streak |
| Spring Cleaning (Bronze) | Objective: Kill a guard with a Broom. |
| Your Wish is Granted (Bronze) | Objective: Throw Money in a Well |
| Fly Like an Eagle (Bronze) | Objective: Jump with the Parachute from the Top of the Castel San Angelo |
| The Gloves Come Off (Bronze) | Objective: Win the highest bet at the Fight Club |
| Mailer Daemon (Bronze) | Objective: Access your Email in the Present |
| Synchronization Established (Bronze) | Objective: Complete an entire Multiplayer Session with at least one kill |
| Needle in a Haystack (Bronze) | Objective: Kill your target while being hidden in a Haystack |
| Strong Closer (Bronze) | Objective: Take the lead 10 seconds before the end of the session and win the game |
| Fast Learner (Bronze) | Objective: Kill your target and escape your assassin in less than 10 seconds |
| Role Model (Bronze) | Objective: Get all the COOP Bonuses in one session |
| Overachiever (Bronze) | Objective: Get a 750 Point Kill or more |
| Abstergo Employee of the Month (Bronze) | Objective: Get Every Bonus at Least Once |
| Ahead of the Curve (Bronze) | Objective: Perform a Double or a Triple Escape |
| Rome in Ruins (Silver) | Objective: Complete DNA Sequence 2 |
| Fixer-Upper (Silver) | Objective: Complete DNA Sequence 3 |
| Principessa in Another Castello (Silver) | Objective: Complete DNA Sequence 4 |
| Fundraiser (Silver) | Objective: Complete DNA Sequence 5 |
| Forget Paris (Silver) | Objective: Complete DNA Sequence 6 |
| Bloody Sunday (Silver) | Objective: Complete DNA Sequence 7 |
| Vittoria Agli Assassini (Silver) | Objective: Complete DNA Sequence 8 |
| Gladiator (Silver) | Objective: Complete LGS 3 [Colosseum] |
| Plumber (Silver) | Objective: Complete LGS 4 [Cloaca Maxima] |
| One-Man Wrecking Crew (Silver) | Objective: Complete LGS 5 [Lateran Palace] |
| Amen (Silver) | Objective: Complete LGS 6 [St. Peter's Basilica] |
| .. .- -- .- .-.. .. ...- . (Silver) | Objective: Solve all of Subject 16's Puzzles |
| Job Skills (Silver) | Objective: During an Open Conflct, kill your target and escape |
| Download Complete (Silver) | Objective: Reach Level 50 |
| A Knife to the Heart (Gold) | Objective: Complete the Game |
| Julius Caeser (Platinum) | Objective: Get Every Trophy |
.