There are 31 Bronze Trophies, 13 Silver Trophies, 2 Gold Trophies, and 1 Platinum Trophy.
| Unlockable: | How to unlock: |
| AI Hacking Specialist (Bronze) | Use AI Hacking 75 times |
| Asari Ally (Bronze) | Complete the majority of the game with the asari squad member |
| Assault Rifle Expert (Bronze) | Register 150 Assault Rifle Kills |
| Barrier Mastery (Bronze) | Use biotic Barrier 75 times |
| Charismatic (Bronze) | Use Charm or Intimidate to resolve an impossible situation |
| Colonial Savior (Silver) | Complete the Bring Down the Sky mission. |
| Completionist (Silver) | Complete the majority of the game |
| Council Legion of Merit (Silver) | Complete Virmire |
| Damping Specialist (Bronze) | Use Damping Field 75 times |
| Distinguished Combat Medal (Silver) | Complete 1 Mass Effect playthrough on the Hardcore difficulty setting. Do not change the setting. |
| Distinguished Service Medal (Silver) | Complete Eden Prime |
| Dog of War (Bronze) | Register 150 organic enemy kills |
| Extreme Power Gamer (Silver) | Reach 60th level with one character |
| First Aid Specialist (Bronze) | Use medi-gel 150 times |
| Geth Hunter (Bronze) | Register 250 synthetic enemy kills |
| Honorarium of Corporate Service (Silver) | Complete Noveria |
| Krogan Ally (Bronze) | Complete the majority of the game with the krogan squad member |
| Lift Mastery (Bronze) | Use biotic Lift 75 times |
| Long Service Medal (Silver) | Complete 2 Mass Effect Playthroughs on any setting |
| Medal of Exploration (Silver) | Land on an uncharted world |
| Medal of Heroism (Silver) | Complete Feros |
| Medal of Honor (Gold) | Complete 1 Mass Effect Playthrough on any setting |
| Medal of Valor (Gold) | Complete 1 Mass Effect playthrough on the Insanity difficulty setting. Do not change the setting. |
| N7 Elite (Platinum) | Acquire all trophies |
| Neural Shock Specialist (Bronze) | Use Neural Shock 75 times |
| Overload Specialist (Bronze) | Use Shield Overload 75 times |
| Paragon (Bronze) | Accumulate 75% of total Paragon points |
| Paramour (Bronze) | Complete any romance subplot |
| Pistol Expert (Bronze) | Register 150 Pistol Kills |
| Power Gamer (Bronze) | Reach 50th level with one character |
| Quarian Ally (Bronze) | Complete the majority of the game with the quarian squad member |
| Renegade (Bronze) | Accumulate 75% of total Renegade points |
| Rich (Silver) | Exceed 1,000,000 Credits |
| Sabotage Specialist (Bronze) | Use Sabotage 75 times |
| Scholar (Silver) | Find all primary Alien: Council Races, Extinct Races and Non-Council Races codex entries |
| Search and Rescue (Bronze) | Locate Dr. T'soni in the Artemis Tau cluster |
| Sentinel Ally (Bronze) | Complete the majority of the game with the Alliance sentinel squad member |
| Shotgun Expert (Bronze) | Register 150 Shotgun Kills |
| Singularity Mastery (Bronze) | Use biotic Singularity 75 times |
| Sniper Expert (Bronze) | Register 150 Sniper Rifle Kills |
| Soldier Ally (Bronze) | Complete the majority of the game with the Alliance soldier squad member |
| Spectre Inductee (Bronze) | Become a Spectre |
| Stasis Mastery (Bronze) | Use biotic Stasis 75 times |
| Tactician (Silver) | Complete playthrough with shield damage greater than health damage |
| Throw Mastery (Bronze) | Use biotic Throw 75 times |
| Turian Ally (Bronze) | Complete the majority of the game with the turian squad member |
| Warp Mastery (Bronze) | Use biotic Warp 75 times |
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