| Unlockable: | How to unlock: |
| Unlock Guy and Kakashi as Playable Characters: | On Easy difficulty setting in Heroes mode finish Survival Exercise stage |
| Unlock Gaara as a Playable Character: | On Normal difficulty setting in Heroes mode finish Leaf Destruction stage. |
| Unlock Jiraiya, Orichmaru, and Tsunade as Playable Charactes: | On Hard difficulty setting in Heroes mode finish The Legendary Sannin stage. |
| Unlock Itachi and Kisame as Playable Characters: | On Insane difficulty setting in Heroes mode finish Atatsuki Returns stage. |
