| Unlockable: | How |
| Captain Falcon(F-Zero) | Clear Classic mode on Normal difficulty in 12 minutes |
| Falco(Star Fox) | Clear 100-Man Brawl |
| Ganondorf(The Legend of Zelda) | Clear Classic mode on Hard difficulty as Link or Zelda or Battle 200 times |
| Jigglypuff(Pokemon) | Have Jigglypuff join you in the Subspace Emissary |
| Lucario(Pokemon) | Have Lucario join you in the Subspace Emissary |
| Luigi(Super Mario Bros.) | Have Luigi join you in the Subspace Emissary |
| Marth(Fire Emblem) | Have Marth join you in the Subspace Emissary |
| Mr Game and Watch(Game and Watch) | Have Mr Game and Watch join you in the Subspace Emissary |
| Ness(EarthBound) | Have Ness join you in the Subspace Emissary |
| R.O.B(Famicom) | Have R.O.B join you in the Subspace Emissary |
| Solid Snake(Metal Gear) | Have Solid Snake join you in the Subspace Emissary |
| Sonic the Hedgehog(Sonic the Hedgehog) | Have Sonic the Hedgehog join you in the Subspace Emissary |
| Toon Link(The Legend of Zelda) | Have Toon Link join you in the Subspace Emissary |
| Star Wolf(Star Fox) | Have Star Wolf join you in the Subspace Emissary |
