| Unlockable: | How to unlock: |
| King Boo | Win 50cc Star Cup in 1st |
| Diddy Kong | Complete all retro 50cc cups 1st |
| Baby Daisy | Get a one star rank on ALL 50cc cups |
| Dry Bones | Win 1st in 100cc Leaf cup |
| Mii (Outfit A- A drivers suit) | Win 1st place in 100cc Special Cup |
| Bowser Jr. | Get a one star rank in all 100cc Retro-Cups |
| Daisy | Win 1st in 150cc Special Cup |
| Dry Bowser | Get a one star ranking on all 150cc Wii-Cups |
| Rosalina | Have a Super Mario Galaxy save file and she will be unlocked randomly |
| Funky Kong | Unlock 4 expert staff ghosts in Time Trials |
| Baby Luigi | Unlock 8 expert staff ghosts in Time Trials |
| Birdo (1st way) | Play Time Trials on 16 different courses |
| Birdo (2nd way) | Win 250 vs matches in Wi-Fi mode |
| Toadette (1st way) | Play Time Trials on all 32 courses |
| Toadette (2nd way) | Win 1000 vs matches in Wi-Fi mode |
| Mii (Outfit B- Mario's overalls) | Unlock all 32 expert staff ghosts in Time Trials |
| Mii (Outfit C- Secret) | 15,000 points in Versus mode (not on Wi-Fi) |
