| Unlockable: | How to unlock: |
| A Big Fan of Lefties (20) | Acquire all enemy officers' left arms. |
| A Lover, Not a Fighter (20) | Successfully complete 10 No Kill battles during story mode. |
| A Walk in the Dark (15) | Complete the sewer sequence in File R-03 without using AR Mode. |
| Amateur Radio Operator (50) | Listen to most of the codec conversations. |
| Analysis Complete (20) | Unlock every VR Mission. |
| Anti-Cyborg Sentiment (5) | Destroy a total of 100 Cyborgs during story mode. |
| Assassin Behind Closed Doors (15) | Successfully complete 30 Ninja Kills during story mode. |
| Assault with a Deadly Weapon (20) | Dismember three enemies during Blade Mode with a single attack. |
| Becoming a Lightning God (50) | Complete story mode on Revengeance difficulty with all S rankings. |
| Chosen by History (30) | Defeat Samuel without taking any damage on Hard difficulty or above. |
| Data Mining (20) | Acquire all data storage devices. |
| Datsu Right (15) | Successfully complete 50 Zandatsus during story mode. |
| Demilitarized Zone (15) | Destroy a total of 100 Heavily Armed Cyborgs during story mode. |
| Dwarf Raiden (15) | Incapacitate all the soldiers in File R-02 using a Dwarf Gekko. |
| Extinction Level Event (20) | Destroy a total of 10 Raptors during story mode. |
| File R-00: Status - Closed (15) | Complete File R-00: Guard Duty. |
| File R-01: Status - Closed (15) | Complete File R-01: Coup d'État. |
| File R-02: Status - Closed (15) | Complete File R-02: Research Facility. |
| File R-03: Status - Closed (15) | Complete File R-03: Mile High. |
| File R-04: Status - Closed (15) | Complete File R-04: Hostile Takeover. |
| File R-05: Status - Closed (15) | Complete File R-05: Escape From Denver. |
| File R-06: Status - Closed (15) | Complete File R-06: Badlands Showdown. |
| File R-07: Status - Closed (15) | Complete File R-07: Assassination Attempt. |
| Genius Destroyer (30) | Defeat Monsoon without taking any damage on Hard difficulty or above. |
| Great Escape (20) | Complete File R-05 in less than 7 minutes. |
| Herpetophobia (20) | Destroy a total of 10 Gekkos during story mode. |
| Humanitarian Assistance (25) | Rescue all the civilians. |
| Ich Liebe Kapitalismus! (20) | Acquire all customization items. |
| Jumping the Shark (20) | Destroy a total of 10 Hammerheads during story mode. |
| Looking Out for the Little Guys (5) | Destroy a total of 30 Dwarf Gekkos during story mode. |
| Love at First Sight (15) | While remotely operating a Dwarf Gekko in story mode, find and communicate with all Dwarf Gekkos. |
| Menace to Society (5) | Cut off the finial at the top of the pagoda in File R-04. |
| No Flash Photography! (15) | Destroy all the Gun Cameras in File R-01. |
| Peekaboo (20) | Discover all of the soldiers hidden in cardboard boxes. |
| Pond Scum (20) | Destroy a total of 5 Vodomjerka during story mode. |
| Prodigal Murderer (30) | Defeat Mistral without taking any damage on Hard difficulty or above. |
| Rip 'Em Apart! (15) | In story mode, kill 100 enemies by cutting them. |
| Silverback (15) | Destroy a total of 10 Mastiffs during story mode. |
| Slider Strike (15) | Destroy a total of 10 Sliders during story mode. |
| Steel Tail (15) | Cut off Metal Gear RAY's metal tail during File R-00. |
| Surprise Attack! (20) | Arrive at the objective in File R-07 without being spotted. |
| Tearing Away the Disguise (20) | Destroy all of the humanoid Dwarf Gekkos during story mode. |
| The Bigger They Are... (10) | Destroy a total of 100 Custom Cyborgs during story mode. |
| The Politics of Silencing Foes (30) | Defeat Sen. Armstrong without taking any damage on Hard difficulty or above. |
| Truly Human (30) | Defeat Sundowner without taking any damage on Hard difficulty or above. |
| Virtually a God (50) | Set the highest score on every VR Mission. |
| VR Master (30) | Complete every VR Mission. |
| What Doesn't Kill You... (20) | In story mode, successfully parry 10 attacks in a row in one minute or less. |
| Wolf Hunter (20) | Destroy a total of 10 Fenrirs during story mode. |
| You Don't Run from Chance (15) | Successfully complete 50 Executions during story mode. |
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